Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity

Circ Res. 2021 Nov 12;129(11):1054-1066. doi: 10.1161/CIRCRESAHA.121.318982. Epub 2021 Oct 4.

Authors

Sarah E Basehore^{1

2}, Samantha Bohlman², Callie Weber², Swathi Swaminathan¹, Yuji Zhang³, Cholsoon Jang⁴, Zoltan Arany⁵, Alisa Morss Clyne²

Affiliations

¹ School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA (S.E.B., S.S.).
² Fischell Department of Biomedical Engineering, College of Engineering, University of Maryland, College Park (S.B., C.W., A.M.C.).
³ Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore (Y.Z.).
⁴ Biological Chemistry, Chao Family Comprehensive Cancer Center, University of California Irvine, Irvine (C.J.).
⁵ Perelman School of Medicine, University of Pennsylvania, Philadelphia (Z.A.).

Abstract

[Figure: see text].

Keywords: acetylglucosamine; cardiovascular diseases; endothelial cells; glycolysis; hemodynamics; hexosamines; nitric oxide.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acetylglucosamine / metabolism*
Animals
Blood Circulation*
Blood Vessels / metabolism
Blood Vessels / physiology
Endothelium, Vascular / metabolism*
Endothelium, Vascular / physiology
Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / metabolism
Glycosylation
Human Umbilical Vein Endothelial Cells / metabolism
Humans
Mice
Mice, Inbred C57BL
N-Acetylglucosaminyltransferases / antagonists & inhibitors
N-Acetylglucosaminyltransferases / metabolism
Nitric Oxide / metabolism
Nitric Oxide Synthase Type III / metabolism*
Phosphorylation

Substances

Nitric Oxide
Nitric Oxide Synthase Type III
N-Acetylglucosaminyltransferases
Ogt protein, mouse
Gfpt1 protein, mouse
Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
Acetylglucosamine

Grants and funding